Table 1 Primary and secondary antibodies used in the study
Species | Type | Dilution | Vendor | Catalog # | Lot # | |
|---|---|---|---|---|---|---|
Primary antibody | ||||||
TH | Chicken | Polyclonal | 1–1000 | Millipore | AB9702 | 3519393 |
NeuN | Mouse | Monoclonal | 1–1000 | Novus | NBP1-92693 | D102247 |
IBA1 | Rabbit | Polyclonal | 1–1000 | Wako | 019-19741 | CAE1308 |
MHC II | Mouse | Monoclonal | 1–500 | BD | BDB554926 | 1137896 |
GFAP | Chicken | Polyclonal | 1–1000 | EnCor | CPCA-GFAP | 7529-5 |
pSyn | Rabbit | Monoclonal | 1–1000 | Abcam | ab51253 | GR3232346-6 |
pTau (AT8) | Mouse | Monoclonal | 1–2000 | ThermoFisher | MN1020 | WJ3381064 |
s100Beta | Rabbit | Monoclonal | 1–1000 | Abcam | Ab54642 | GR3215095-29 |
s100Beta | Rabbit | Monoclonal | 1–1000 | Novus | NBP2-53188 | 6285-4P10803 |
s100Beta | Mouse | Monoclonal | 1–1000 | ProteinTech | 6616-Ig | 10004814 |
Secondary antibody | ||||||
Alexa 488 anti-rabbit | Goat | Polyclonal | 1–1000 | Invitrogen | A32721 | UD282059 |
Alexa 555 anti-chicken | Goat | Polyclonal | 1–1000 | Invitrogen | A21437 | 2352144 |
Alexa 647 anti-mouse | Goat | Polyclonal | 1–1000 | Invitrogen | A32728 | UD279293 |
IRDye 680RD anti-rabbit | Donkey | Polyclonal | 1–10,000 | LICOR | 925-68073 | C90129-11 |
IRDye 800CW anti-mouse | Donkey | Polyclonal | 1–10,000 | LICOR | 926-32212 | D20126-04 |
HRP anti-rabbit | Goat | Polyclonal | 1–1000 | Cell signaling | 7074P2 | 31 |
HRP anti-mouse | Goat | Polyclonal | 1–2000 | Invitrogen | 62-6520 | UA281872 |