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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Single-cell spatial proteomic imaging for human neuropathology

Fig. 1

Workflow and Features Extracted from MIBI-TOF Spectral Images. a MIBI-TOF experimental workflow begins with the staining of FFPE brain regions (e.g., hippocampus, substantia nigra, striatum, locus coeruleus, medulla oblongata, cerebellum) on conductive gold sides, and imaging with a panel of 36 antibodies, each labeled with a unique elemental mass tag. Stained tissue sections were rasterized and resulted in a 36-dimensional image revealing protein expression at unique spatial coordinates in a selected field of view (FOV). MIBI-TOF generates data to quantify and visualize tissue architecture, which allows analysis of global protein expression and distribution, discretization into biological units for exploring marker relationships within the biological units, and investigation of distance relationships and neighborhoods among these biological units. b–e Montage of 196 tiled FOVs MIBI-TOF image from a post-mortem FFPE archival human hippocampus from an individual with AD dementia (ADD). Each tile is a 500μm2 spectral image acquired at low-resolution scan resolving ~ 1 µm2/pixel. Representative 11 out or 36 spectral images were pseudo-colored and overlaid to show the spatial distribution and expression of structural and molecular markers. Abbreviations: DG, Dentate Gyrus; CA, Conus Ammonis; Lmol, molecular layer

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