Fig. 2

Cell viability assay valuation of the predicted drugs. Triptolide (A), triamterene (B) and mycophenolate mofetil (C) in DIPG cell lines (SF8628, SU-DIPG-IV) as well as control NHA cells. Left: Graphs showing the proliferation response of normal human astrocytes (NHA) and DIPG cell lines (SU-DIPG-IV, SF8628), to increasing concentrations of each drug. Values shown are the average [mean ± standard deviation (SD)] from triplicate samples for each incubation condition. Right: Dot plot representation of IC50 values shown are the average (mean ± SD) from triplicate samples for each cell lines. Statistical analysis was performed using a two-tailed unpaired t-test: triptolide, NHA versus SU-DIPG-IV, *P < 0.05; NHA versus SF8628, *P = 0.05; triamterene, NHA versus SU-DIPG-IV, *P < 0.05; NHA versus SF8628, *P < 0.05; MMF, NHA versus SU-DIPG-IV, ***P < 0.001; NHA versus SF8628, **P < 0.01