Fig. 3

Immunohistochemical analysis with MRP-14 of brain sections obtained from non-infected wt mice (a–c), S. pneumoniae-infected wt mice (d–f) and S. pneumoniae-infected Serpinb2-deficient mice (g–i). Brain sections were obtained at 48 h after intracisternal application of PBS or S. pneumoniae (3 mice per group). The sections were stained with anti-murine MRP-14 antibody and counterstained with hematoxylin–eosin. Mice lacking Serpinb2 showed enhanced MRP-14 staining in the brain parenchyma (g, h) and to a lesser degree also in infiltrates in the leptomeningeal space (i), compared to wt mice subjected to PM (d–f). The scale bar indicates 200 µm in length