Fig. 1

An overview of hiPSCs as a tool to investigate schizophrenia in vitro models. Fibroblasts, blood cells or keratinocytes from control subjects and/or schizophrenia patients are collected and reprogrammed to hiPSCs—some of which may be gene corrected and used as isogenic controls. The established hiPSCs can then be differentiated into the cells of interest, for example, neuronal progenitors cells (NPCs), neurons, glia or used for 3D or mixed cultures to model schizophrenia in vitro. Several readout methods such as single cell RNA (sc-RNA) sequencing, different cellular assays, cell imaging, mass spectrometry and electrophysiology can be used to investigate cell cultures in vitro