Fig. 2

Example spectral images of BSB- and MCAAD-3-stained 5xFAD mouse and human AD brain sections. (A,B) With 100 nM BSB, plaques and neurofibrillary tangles (in the human, arrowheads) were well seen over a dark background. (C) Average spectra from the three features exhibited obvious differences, with tangles blue-shifted compared to plaques. (D) Phasor plot from the spectral image in B shows characteristic peaks reflective of plaques (labeled in B with asterisks) and tau tangles (labeled in B with arrowheads). (E,F) Staining of 5xFAD and human AD sections with 25 nM MCAAD-3 revealed similar patterns of amyloid plaque deposition in both mouse and human tissue with similar degrees of spectral shift between species as was the case with BSB (G) In contrast to BSB however, tangles were barely seen with MCAAD-3 underscoring differences in the ability of various amyloid probes to detect different amyloid deposits. (H) In contrast to BSB, the phasor plot of the human AD MCAAD-3 image exhibited a single narrow peak that corresponded to bright amyloid β deposits, with no obvious tangles. Scale bars: 50 μm