Fig. 1
Enrichment analysis of our synaptic fractions. A Schematic diagram shows experimental workflow. Synaptically-enriched fractions were collected using BA9 and BA4 brain areas from both control and ALS patients. Synaptic protein fractions were Tandem Mass Tag (TMT) labelled and analysed by liquid chromatography, mass spectrometry-based quantification and identification (LC–MS/MS). Data was analysed using MaxQuant quantitative proteomics software. B Schematic showing our experimental pooling strategy. Each coloured rectangle represents one experimental group, there are 10 in total. The number of individual samples in each pool is indicated. C The identified 5348 unique proteins demonstrate a 99% overlap with previously published human proteomics data. D, E Bioinformatics analysis using ShinyGo enrichment analyser with protein IDs show the top 30 KEGG pathways (D) and plotted based on fold enrichment and gene counts/pathway (dot size), colorized by -log10(FDR). Colorized bar graphs by gene counts show (E) the top 15 enriched biological terms in Gene Ontology (GO) clusters