Fig. 9From: Müller cell degeneration and microglial dysfunction in the Alzheimer’s retinaLayer-wise cross-sectional colocalization profile of GFAP labelled astrocytes with Aβ. Left panel illustrate age-matched controls (N = 7). Right panel illustrate AD donors (N = 5). The primary antibody against GFAP was visualized using a FITC-labelled secondary antibody. The primary antibody against Aβ was visualized using a Cy3-labelled secondary antibody. Green bars represent the percentage of Aβ positive pixels among FITC (GFAP) -positive pixels. Black bars represent the percentage of Aβ positive pixels among FITC (GFAP) -negative pixels. Colocalization is defined by FITC-positive pixels having a significantly higher percentage of Aβ positivity than FITC-negative pixels. Aβ colocalization with GFAP is calculated in each retinal layer and plotted on the x-axis. One out of 12 geo-layers in controls (A) and 3 out of 12 geo-layers in AD donors (B) showed significant Aβ-GFAP colocalization. * represent p < 0.05. ** represent p < 0.01. Error bars = Standard ErrorBack to article page