Fig. 8
From: Müller cell degeneration and microglial dysfunction in the Alzheimer’s retina
Representative immunofluorescence images of retinal punches. A, C, E Post-mortem human retina samples were processed from AD donors (Mean age = 75.3) (N = 3). B, D, F Post-mortem human retina samples were processed from controls (Mean age = 70.7) (N = 3). Tissues underwent immuno-histochemistry staining for Aβ (red), and either GFAP for astrocytes/Müller cells (green), IBA-1 for microglia (green) or TUBB3 for neuronal microtubules (green). Aβ (red) immunofluorescence was evident within what was likely the retinal ganglion cells (asterisk) and in the neuropil and extracellular spaces (arrows). Stronger Aβ immunoreactivity (red) was observed intracellularly in retinal ganglion cells and in the neuropil of AD donors compared to controls. Note abnormal nodular appearance of axonal profiles (green TUBB3) in AD (E). Scale bar = 20 µm. Error bars = Standard Error