Fig. 7
From: Müller cell degeneration and microglial dysfunction in the Alzheimer’s retina
Representative immunofluorescence images of retinal cross-sections. A, C, E Post-mortem human retina samples were processed from AD donors (Mean age = 78.6) (N = 5). B, D, F Post-mortem human retina samples were processed from controls (Mean age = 76.8) (N = 6). Tissues underwent immunohistochemistry staining for Aβ (red), nuclei (blue), and either GFAP for astrocytes/Müller cells (green) or IBA-1 for microglia (green) or TUBB3 for neuronal microtubules (green). Aβ immunofluorescence was evident within what is likely the retinal ganglion cells (asterisk) and the neuropil and extracellular spaces (arrowheads). Double labelling is seen (arrows). Stronger Aβ immunoreactivity (red) was observed intracellularly in retinal ganglion cells and in the neuropil of AD donors compared to controls. Scale bar = 25 µm