Fig. 10

Layer-wise cross-sectional colocalization profile of IBA-1 labelled microglia with Aβ. Left panel illustrate age-matched controls (N = 7). Right panel illustrate AD donors (N = 5). The primary antibody IBA-1 was visualized using a FITC-labelled secondary antibody. The primary antibody against Aβ was visualized using a Cy3-labelled secondary antibody. Green bars represent the percentage of Aβ positive pixels among FITC (IBA-1) -positive pixels. Black bars represent the percentage of Aβ positive pixels among FITC (IBA-1) -negative pixels. Colocalization is defined by FITC (IBA-1) -positive pixels having a significantly higher percentage of Aβ positivity than FITC-negative pixels. Aβ colocalization with IBA-1 is calculated in each retinal layer and plotted on the x-axis. Ten out of 12 geo-layers in controls (A) and 4 out of 12 geo-layers in AD donors (B) showed significant Aβ-IBA-1 colocalization. * represent p < 0.05. **represent p < 0.01. *** represent p < 0.001. Error bars = Standard Error