Fig. 4

Modification of inflammation network following Treg administration. Volcano Plots showing fold changes vs. p-values of nCounter Mouse Neuroinflammation panel in A 5xFAD-WT vs. WT-WT; 73 immune related genes were upregulated (green dots) and 5 genes were down regulated (blue dots) in 5xFAD-WT (n = 4 in each groups). B Compared with WT-Rag2KO, 95 genes were upregulated (green dots) and 7 genes were down-regulated (blue dots) in 5xFAD-Rag2KO. The 10 most upregulated genes and all down-regulated genes are labeled in the figures. C Treg-treated 5xFAD-Rag2KO vs. 5xFAD-Rag2KO; the pathologic upregulation of inflammation related genes in 5xFAD-Rag2KO were modified following Treg administration. D The network representation and subcellular assignment of the enriched pathway in Treg-treated 5xFAD-Rag2KO vs. untreated 5xFAD-Rag2KO. The enriched network were centered in down-regulation of pro-inflammatory cytokines (IL1A&B, IL6, Tnfa, IFNγ), complement activation (C1qa, C1qb, C1qc, C4a/b), toll like receptors (Tlr3, Tlr4 and Tlr7), myeloid activation markers (CD14, Tyrobp,Trem2) and intra-nuclear binding motifs of interferon-regulatory factors (IRF3 and IRF7)