Fig. 3

Treg administration reduces number of reactive astrocytes. Representative images of GFAP positive-reactive astrocytes (green) and Aβ 1–42 positive plaques (red) in the dentate gyrus (DG) (A) and frontal cortex (FC) (F) of WT-WT, 5xFAD-WT, 5xFAD-Rag2KO and Treg-treated 5xFAD-Rag2KO (n = 6 per group, sex balanced). B–E Quantification of the number of GFAP⁺ reactive astrocytes in the DG and FC; number of GFAP⁺ reactive astrocytes was increased in 5xFAD-WT mice, compared to WT-WT. Lack of adaptive immune system in 5xFAD-Rag2KO had no effect on the number of GFAP⁺ reactive astrocytes, compared to 5xFAD-WT. Decreased number of total and plaque-associated GFAP⁺ reactive astrocytes in 5xFAD-Rag2KO were noted following Treg administration. Numbers shown as averages ± SEM with one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Scale bar, 100 μm