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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Tau seeding in cases of multiple sclerosis

Fig. 1

Tau biosensors detect tau seeds in homogenates from MS brains. A Schematic of the FRET-based tau biosensor cell assay for the detection of tau seeds. HEK293 cells express tau repeat-domain with mCerulean and mClover tags that produce FRET upon close association. Lipofectamine-mediated transduction of exogenous tau seeds initiates aggregation of tau-RD constructs, which is quantified via FRET flow cytometry. B Seeding was detected in 6/8 MS brains with either RRMS (AC) or SPMS (D–H) based on immunopurification of tau seeds using MD3.1 antibody. Columns represent the mean % FRET positive cells of six technical replicates (dots). Statistical significance was determined by performing one-way ANOVA followed by Dunnett’s multiple comparisons testing of all samples compared against Lipofectamine treated negative controls, **p < 0.01, ****p < 0.0001. Errors bars = S.D

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