Fig. 3

Elevated phosphorylation of β-catenin pSer552, AHSG pSer138, and ITIH2 pSer60 in recurrent NF-PitNETs. Phosphoprotein profiles in recurrent NF-PitNET showed significant increase in phosphorylation of β-catenin pSer552 (a), Alpha-2-HS-glycoprotein (AHSG) pSer138 (b), and Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) pSer60 (c) in recurrent (R) NF-PitNETs subgroup compared to invasive (I) and non-invasive/non-recurrent (NI/NR). (d) Graphical representation of the Ion Score (a measure of how well the observed MS/MS spectrum matches the stated peptide) shows highest Ion Score for β-catenin pSer552 compared to AHSG pSer138 and ITIH2 pSer60. (e) Graphical representation of XCorr (cross correlation). Values above 2.0 indicate of a good fit of experimental peptide fragments to theoretical spectra. XCorr was significantly high for β-catenin pSer552 compared to other phophoproteins. Abbreviations: NI/NR, non-invasive/non-recurrent; I, invasive; R, recurrent. A two-tailed unpaired Student’s t-test was performed *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001