Fig. 2
Heat map reveals differential clustering of phosphoproteins among NF-PitNET subgroups. a The fold change values of 30 differentially regulated phosphoproteins were grouped by an unsupervised hierarchical clustering using Pearson’s correlation distance and average linkage. Proteins with elevated phosphorylation levels are indicated by red while decrease in phosphorylation is shown by green. Each row represents a phosphoprotein (phosphosites shown in brackets) and each column a NF-PitNET subgroup: invasive (I), recurrent (R) and non-invasive/non-recurrent (NI/NR). b Volcano plot, obtained from quantitative mass spectrometry, indicating differentially phosphorylated protein in recurrent (R) NF-PitNETs. The Log2 fold change indicates the mean phosphorylation level of proteins. Each dot represents a phosphoprotein. Multiple-t test shows significant hyperphosphorylation of β-Catenin (p = 0.000016), AHSG (p = 0.0006), and ITIH2 (p = 0.001) in recurrent NF-PitNETs