Fig. 1

Overview of the phosphoproteome of NF-PitNETs. a Graphical quantification of the phosphopeptides fold change among NF-PitNETs subgroups: invasive (I, green), recurrent (R, purple) and non-invasive/non-recurrent (NI/NR, pink). Statistical analysis reveals differentially expressed phosphopeptides between I, R, and NI/NR subgroups. Note that fold change is compared to non-invasive/non-recurrent NF-PitNETs subgroup. b Bar graph indicating the overall proportion of phosphoserine, phosphothreonine and phosphotyrosine sites in the NF-PitNETs phosphoproteome. Overview of phosphorylation sites per amino acid shows that Ser is the most phosphorylated amino acid followed by threonine (Thr) and tyrosine (Tyr). c Bar graph representing the number of phosphopeptides carrying either a single phosphosite, double phosphosites, triple and more than three phosphosites. d-e The phosphorylation of Ser and Thr is significantly upregulated in recurrent compared to invasive and NI/NR f No differences were found in the phosphorylation status of Tyr residues among the I and R, NF-PitNETs subgroups. Abbreviations: I, invasive; R, recurrent; Ser, serine; Thr, threonine; Tyr, tyrosine. d–f Student’s t-test from experimental triplicates, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001