Antibody or reagent | Source species | Dilution | Application | Source | Catalog. # |
---|---|---|---|---|---|
Primary antibody | |||||
Fcrls mAb | Rat | 1:200 | FCM | Butovsky Lab | N/A |
CD11b-PeCy7 | Rat | 1:300 | FCM | eBioscience | 50-154-54 |
Ly-6C-PerCP/Cy5.5 | Rat | 1:300 | FCM | BioLegend | 123,012 |
Clec7a mAb | Rat | 1:100 1:50 | IF-CS IF-FL | InvivoGen | Mabg-mdect |
TMEM119 mAb | Rabbit | 1:500 1:200 | IF-CS IF-FL | Synaptic Systems | 400,011 |
Alexa Fluor 488-conjugated tomato lectin | Lycopersicon esculentum | 1:200 1:100 1:100 | IF-CS IF-FL IF-RMVI | DyLight | DL-1174 |
Galectin-3 pAb | Goat | 1:100 1:50 1:1000 | IF-CS IF-FL WB | R&D Systems | AF1197 |
IBA1 | Rabbit | 1:100 1:50 | IF-CS IF-FL | Fujifilm Wako Chemicals | 019-19,741 |
IBA1 | Goat | 1:100 1:50 1:1000 | IF-CS IF-FL WB | Novus Biologicals | NB100-1028 |
Aβ42 (12F4) mAb | Mouse | 1:200 | IF-CS | BioLegend | 805,501 |
P2ry12 | Rabbit | 1:200 1:500 | IF-CS WB | Butovsky Lab | N/A |
APOE pAb | Goat | 1:200 | IF-CS | Millipore Sigma | AB947 |
phospho-NF-κB p65 Ser536 mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 3033S |
NF-κB p65 mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 8242S |
TNF-α mAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 11948S |
IL-1β mAb | Mouse | 1:1000 | WB | Cell Signaling Technology | 12242S |
EIF3c pAb | Rabbit | 1:1000 | WB | Cell Signaling Technology | 2068S |
NDUFA6 pAb | Rabbit | 1:1000 | WB | Thermo Fisher Scientific | PA5-97,259 |
NDUFA10 pAb | Rabbit | 1:1000 | WB | Thermo Fisher Scientific | PA5-21,474 |
Spp1 | Goat | 1:100 1:20 1:500 | IF-CS IF-FL WB | R&D Systems | AF808 |
GFAP | Rat | 1:50 | IF-FL | Invitrogen | 13–0300 |
4G8 mAb | Mouse | 1:200 | IF- RMVI | Biolegend | 800,701 |
Aβ40 (11A50-B10) mAb | Mouse | 1:200 | IF- RMVI | Biolegend | 805,401 |
Zonula Occluden-1 pAb | Rabbit | 1:50 | IF- RMVI | Thermo Fisher Scientific | 61–7300 |
Claudin-1 pAb | Rabbit | 1:75 1:1000 | IF- RMVI WB | Thermo Fisher Scientific | 51–9000 |
MMP-9 | Goat | 1:800 | WB | R&D Systems | AF909 |
Secondary antibody | |||||
APC anti-rat IgG clone Poly4054 | Goat | 1:300 | FCM | BioLegend | |
Cy2 (anti-Rabbit, anti-goat, anti-rat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
Cy3 (anti-rabbit, anti-goat, anti-rat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
Cy5 (anti-mouse, anti-rabbit, anti-rat, anti-goat) | Donkey | 1:200 | IF | Jackson ImmunoResearch Laboratories | |
Peroxidase Donkey Anti-Rabbit IgG (H + L) | Donkey | 1:10,000 | WB | Jackson ImmunoResearch Laboratories | |
Peroxidase Donkey Anti-Mouse IgG (H + L) | Donkey | 1:10,000 | WB | Jackson ImmunoResearch Laboratories | |
Peroxidase Donkey Anti-Goat IgG (H + L) | Donkey | 1:10,000 | WB | Jackson ImmunoResearch Laboratories |