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Table 1 List of antibodies

From: Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer’s-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia

Antibody or reagent

Source species

Dilution

Application

Source

Catalog. #

Primary antibody

Fcrls mAb

Rat

1:200

FCM

Butovsky Lab

N/A

CD11b-PeCy7

Rat

1:300

FCM

eBioscience

50-154-54

Ly-6C-PerCP/Cy5.5

Rat

1:300

FCM

BioLegend

123,012

Clec7a mAb

Rat

1:100

1:50

IF-CS

IF-FL

InvivoGen

Mabg-mdect

TMEM119 mAb

Rabbit

1:500

1:200

IF-CS

IF-FL

Synaptic Systems

400,011

Alexa Fluor 488-conjugated tomato lectin

Lycopersicon esculentum

1:200

1:100

1:100

IF-CS

IF-FL

IF-RMVI

DyLight

DL-1174

Galectin-3 pAb

Goat

1:100

1:50

1:1000

IF-CS

IF-FL

WB

R&D Systems

AF1197

IBA1

Rabbit

1:100

1:50

IF-CS

IF-FL

Fujifilm Wako Chemicals

019-19,741

IBA1

Goat

1:100

1:50

1:1000

IF-CS

IF-FL

WB

Novus Biologicals

NB100-1028

42 (12F4) mAb

Mouse

1:200

IF-CS

BioLegend

805,501

P2ry12

Rabbit

1:200

1:500

IF-CS

WB

Butovsky Lab

N/A

APOE pAb

Goat

1:200

IF-CS

Millipore Sigma

AB947

phospho-NF-κB p65 Ser536 mAb

Rabbit

1:1000

WB

Cell Signaling Technology

3033S

NF-κB p65 mAb

Rabbit

1:1000

WB

Cell Signaling Technology

8242S

TNF-α mAb

Rabbit

1:1000

WB

Cell Signaling Technology

11948S

IL-1β mAb

Mouse

1:1000

WB

Cell Signaling Technology

12242S

EIF3c pAb

Rabbit

1:1000

WB

Cell Signaling Technology

2068S

NDUFA6 pAb

Rabbit

1:1000

WB

Thermo Fisher Scientific

PA5-97,259

NDUFA10 pAb

Rabbit

1:1000

WB

Thermo Fisher Scientific

PA5-21,474

Spp1

Goat

1:100

1:20

1:500

IF-CS

IF-FL

WB

R&D Systems

AF808

GFAP

Rat

1:50

IF-FL

Invitrogen

13–0300

4G8 mAb

Mouse

1:200

IF- RMVI

Biolegend

800,701

40 (11A50-B10) mAb

Mouse

1:200

IF- RMVI

Biolegend

805,401

Zonula Occluden-1 pAb

Rabbit

1:50

IF- RMVI

Thermo Fisher Scientific

61–7300

Claudin-1 pAb

Rabbit

1:75

1:1000

IF- RMVI

WB

Thermo Fisher Scientific

51–9000

MMP-9

Goat

1:800

WB

R&D Systems

AF909

Secondary antibody

APC anti-rat IgG clone Poly4054

Goat

1:300

FCM

BioLegend

 

Cy2 (anti-Rabbit, anti-goat, anti-rat)

Donkey

1:200

IF

Jackson ImmunoResearch Laboratories

 

Cy3 (anti-rabbit, anti-goat, anti-rat)

Donkey

1:200

IF

Jackson ImmunoResearch Laboratories

 

Cy5 (anti-mouse, anti-rabbit, anti-rat, anti-goat)

Donkey

1:200

IF

Jackson ImmunoResearch Laboratories

 

Peroxidase Donkey Anti-Rabbit IgG (H + L)

Donkey

1:10,000

WB

Jackson ImmunoResearch Laboratories

 

Peroxidase Donkey Anti-Mouse IgG (H + L)

Donkey

1:10,000

WB

Jackson ImmunoResearch Laboratories

 

Peroxidase Donkey Anti-Goat IgG (H + L)

Donkey

1:10,000

WB

Jackson ImmunoResearch Laboratories

 
  1. FCM Flow cytometry, IF immunofluorescence, CS cross-section, FL flat mount, RMVI retinal microvascular isolation, WB western blot, pAb polyclonal antibody, mAb monoclonal antibody, N/A not applicable