Table 4 Primary antibodies used in the present study
From: Tau seeding activity in various regions of down syndrome brain assessed by two novel assays
Antibody | Type | Species | Specificity | Source/reference (catalog) |
|---|---|---|---|---|
43D | Mono- | M | Tau (8–16) | In-house/BioLegend (816,601)[42] |
77G7 | Mono- | M | Tau (316–355) | In-house/BioLegend (816,701)[42] |
R134d | Poly- | R | Total tau | In-house[42] |
AT8 | Mono- | M | pSer202/Thr205-tau | Thermo Scientific (MN1020) |
Anti-pS199 | Poly- | R | pSer199-tau | Invitrogen (44-734G) |
Anti-pT205 | Poly- | R | pThr205-tau | Invitrogen (44-738G) |
Anti-pT212 | Poly- | R | pThr212-tau | Invitrogen (44-740G) |
Anti-pS214 | Poly- | R | pSer214-tau | Invitrogen (44-742G) |
Anti-pT217 | Poly- | R | pSer217-tau | Invitrogen (44–744) |
AT180 | Mono- | M | pThr231-tau | Invitrogen (MN1040) |
12E8 | Mono- | M | pSer262/Ser356-tau | Dr. D. Schenk |
PHF-1 | Mono- | M | pSer396/Ser404-tau | Dr. Peter Davies [23] |
R145d | Poly- | R | pSer422-tau | In-house [42] |
Anti-HA | Mono- | M | HA | Sigma (H9658) |
RD3 | Mono- | M | 3R-tau | Millipore (05–803) |
Anti-GAPDH | Poly- | R | GAPDH | Sigma (G9545) |