Fig. 6
From: Tau seeding activity in various regions of down syndrome brain assessed by two novel assays
Tau seeding activity in different regions of DS brain assessed by the seeded-tau aggregation assay in cultured cells. a, b HEK-293FT cells overexpressing HA-tau151-391 were treated with brain extracts of various regions from DS and control cases using the same amount of tau for 42 h. RIPA-soluble and -insoluble taus were analyzed by Western blots (a). Levels of RIPA-insoluble tau are presented as scatter plots with mean ± SD (b). c, d Levels of RIPA-insoluble tau analyzed by immuno-dot blots developed with anti-HA are presented as scatter plots with mean ± SD (c) or as across various brain regions (d). *P < 0.05, **P < 0.01, ****P < 0.0001