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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Tau seeding activity in various regions of down syndrome brain assessed by two novel assays

Fig. 4

Seeding activity of proteopathic tau in DS brain extracts found by tau capture assay. a. Representative image of tau capture assay. Brain extracts of temporal cortex (TC), frontal cortex (FC), occipital cortex (OC), corpus callosum (CC), and cerebellar cortex (CBC) from DS and control cases were applied onto a NC membrane in the same amount of protein and subjected to tau capture assay by incubating the membrane with HEK-293FT/HA-tau151-391 cell lysate. The captured tau was developed with anti-HA followed by HRP-2nd-Ab and ECL. b, c. The levels of captured tau are presented as a scatter plots with mean ± SD (b) and heatmap (c). **P < 0.01; ***P < 0.001; ****P < 0.0001. d. Seeding activity crossing various brain regions assessed by tau capture assay in DS cases

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