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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Purkinje cell vulnerability induced by diffuse traumatic brain injury is linked to disruption of long-range neuronal circuits

Fig. 2

Traumatic brain injury causes early formation of axonal varicosities in the cerebellar white matter and impairment of motor function. a,c) Schematic diagram of spatial distribution of axonal varicosities in the different regions of cerebellar white matter. (1) In long-range pathways that terminates as mossy fibers and (2) in the region of cerebellar nuclei (2) b Confocal images of varicosities along GFP + axons (green) axons in green boxed area (1) in the cFPI injured Thy1-GFP-M mice. d Confocal images of varicosities along GFP + axons (green) axons in green-boxed area (2) in the cFPI injured mice. There were only few or no varicosities in the sham-injured mice in the regions marked by the green boxes in a and c. GFP signals are inverted into grey scale. Red arrows point to axonal varicosities. Scale bar 20 μm. e Schematic diagram of mice while performing the beam walk test. f Reduced limb coordination of cFPI mice in the beam walk test. Percentage of hindlimb slips in the non-injured, sham and cFPI mice at 1 week and 4 weeks after the injury. g The average time to cross the beam significantly decreases in the cFPI at 7 days after the injury (**P < 0.001; (*P < 0.01, n = 5 (non-injured, naïve), n = 5 (Sham), n = 5 (cFPI), Kruskal–Wallis followed by Dunn’s post hoc test)

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