Fig. 7

Photomicrographs of cannabinoid receptor 1 (CB1) expression in neurons and astrocytes. Confocal microscopy images of cortical brain sections triple labelled for CB1 (red), NeuN (green) and GFAP (blue) in WT (a–d, i and j) and α-DB KO mice (d–h, k and l). 3D reconstruction of confocal images (i–l) shows direct contact between CB1-positive staining and NeuN-positive cells (arrow heads), while little to no colocalization is noted between CB1 and GFAP (arrows). Images in j and l are magnifications of shaded areas in i and k. Scale bar d, h, i and k = 20 μm, j and l = 5 μm