Fig. 4

Intake of food, water and milkshake during and after food restriction. Food-restricted mice were allowed to consume food pellets and water (a) or food pellets and milkshake (b) for 1 h. WT and α-DB KO mice consumed the same amount of food, water and milkshake during this period. Intake of food and water did not differ between WT and KO animals allowed ad libitum access (c and d). Fully fed α-DB KO mice drank significantly more milkshake than WT mice over a 24-h period (e). Body weight at the start (12 weeks old) and end (32 weeks old) of the experiment did not differ between WT and KO mice, although both groups gained a significant amount of weight over time (f). KO mice showed a trend towards greater weight gain between the start and end of the experiment compared to WT animals (f). n = 12/group. For a–e, p > 0.05, two-way repeated measures ANOVA. For f, ****p < 0.0001, two-way repeated measures ANOVA with Sidak’s post-hoc