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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Protein citrullination marks myelin protein aggregation and disease progression in mouse ALS models

Fig. 6

Citrullinated proteins accumulate in astrocytes and mark both axons and protein aggregates in the spinal cord white matter. A, D Double immunofluorescence staining for GFAP and citrullinated proteins in SOD1G93A and PFN1C71G mice, respectively. Arrows point to astrocytic processes that are positive for GFAP and citrullinated proteins. Arrowheads point to GFAP-negative but citrulline-positive aggregate-like structures. B Quantification of fluorescent intensity of GFAP staining in A. C Quantification of fluorescent intensity of citrullinated protein signal in areas that overlap with GFAP staining in A. E Quantification of fluorescent intensity of GFAP staining in D. Quantification of fluorescent intensity of citrullinated protein signal in areas that overlap with GFAP staining in D. G, J Double immunofluorescence staining for NF-L and citrullinated proteins in SOD1G93A and PFN1C71G mice, respectively. Arrows point to NF-L-negative and citrullinated protein-positive astrocytic processes. Filled arrowheads point to NF-L-negative but citrullinated-protein-positive aggregates. Unfilled arrowheads point to NF-L-positive and citrullinated protein-positive axons. H Quantification of fluorescent intensity of NF-L staining in G. I Quantification of fluorescent intensity of citrullinated protein signal in areas that overlap with NF-L staining in G. K Quantification of fluorescent intensity of NF-L staining in J. L Quantification of fluorescent intensity of citrullinated protein signal in areas that overlap with NF-L staining in J. MO Number, area, and staining intensity of NF-L-negative and citrulline-positive aggregates, respectively, in SOD1G93A mice. PR Number, area, and staining intensity of NF-L-negative, citrulline-positive aggregates, respectively, in PFN1C71G mice. The ages of nTg mice are as described in Fig. 2. For quantification in B, C, E, F, H-R, n = 6–7 in all groups. Statistics: One-way ANOVA with Bonferroni post-hoc test for comparison among the different groups. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Comparison bars lacking asterisks (*) indicate a lack of significant difference

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