Fig. 5
From: Protein citrullination marks myelin protein aggregation and disease progression in mouse ALS models
The increase of citrullinated proteins and PAD2 are correlated with regions of most motor neuron degeneration in the CNS. A, B Western blots of citrullinated proteins in cervical spinal cord (CSC), brainstem (BS), cerebellum (CB) and cortex (CTX) at paralysis stage in SOD1G93A and PFN1C71G mice, respectively. A CSC sample from paralysis stage was used as the positive control (+ Ctrl) in the cerebellum and cortex blots. C, D Quantification of citrullinated protein levels in A, B respectively. E, F Western blots of PAD2 protein in CSC, BS, CB, and CTX at paralysis stage in SOD1G93A and PFN1C71G mice, respectively. G, H Quantification of PAD2 protein levels in E, F respectively. n = 4 in each group. Statistics: unpaired t test for comparing transgenic mice with their age matched controls. *p < 0.05; **p < 0.01; ***p < 0.001. Unmarked pairs are not significantly different