Fig. 4
From: Protein citrullination marks myelin protein aggregation and disease progression in mouse ALS models
PAD2 expression is increased progressively in astrocytes but decreased in neurons in the spinal cord. A Immunohistochemistry staining of PAD2 in ventral horn spinal cords of SOD1G93A and PFN1C71G mice. Filled arrowheads point to PAD2-negative neurons, unfilled arrowheads point to PAD2-positive neurons, arrows point to PAD2-positive astrocytes. B Double immunofluorescence staining for GFAP and PAD2 in ventral horn in SOD1G93A mice. C, D Quantification of fluorescent intensity of GFAP and PAD2, respectively, in B. E Double immunofluorescence staining for GFAP and PAD2 in ventral horn in PFN1C71G mice. F, G Quantification of fluorescent intensity of GFAP and PAD2, respectively, in E. H Double immunofluorescence staining for NeuN and PAD2 in ventral horn in SOD1G93A mice. I Quantification of fluorescent intensity of PAD2 staining in NeuN-positive cells in H. J Double immunofluorescence staining for NeuN and PAD2 in ventral horn in PFN1C71G mice. K Quantification of fluorescent intensity of PAD2 staining in NeuN-positive cells in J. Note that the PAD2 staining was overexposed in H, J to ensure the visualization of weak signals in neurons. The ages of nTg mice are as described in Fig. 2. n = 8 in all groups. Statistics: one-way ANOVA with Bonferroni post-hoc test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Comparison bars without (*) sign indicate not significant