Fig. 4
From: LATE-NC aggravates GVD-mediated necroptosis in Alzheimer’s disease
pMLKL and pTau severity as well as neuronal loss are increased in AD cases with LATE-NC. a Local accumulation of phosphorylated tau and MLKL is observed in ADTDP+. DAB immunohistochemical staining of pTDP-43 (S409/S410), pTau (S202/T205) and pMLKL (S358) in the CA1-subiculum field of a control, ADTDP− and ADTDP+ case (cases 7, 16 and 21 are displayed, see Table 2). Scale bars = 50 µm. b ADTDP+ cases display decreased neuronal density compared to controls and ADTDP− and controls. Quantitative data representing the number of total neurons per mm2 per group in the CA1 subfield of the hippocampus. Quantification of the number of CA1 positive neurons for c pTDP-43 d pMLKL and e pTau. ADTDP+ cases display significantly higher severity of pMLKL and pTau lesions. Data are presented as mean ± SEM. N = 27 (controls = 9, ADTDP− = 8, ADTDP+ = 10). f Partial Spearman correlation matrix controlled for age and multiple comparisons (Holm-Bonferroni test) in this cohort confirms that the accumulation of pTDP-43, pMLKL and pTau pathologies are significantly correlated with neuronal density in the CA1, AβMTL phase and Braak NFT stage. Supporting images showing overviews of the whole hippocampus of cases 12, 16, 21 and 22 (Table 2) stained with pMLKL can be found in the public repository BioImage Archive, with the hyperlink: https://www.ebi.ac.uk/biostudies/studies/S-BIAD514?key=475a3bbe-6fc9-476e-8e45-6429422b85bf