Table 1 Positive staining patterns of lymphatic endothelial cell (LEC) markers in the saccular intracranial aneurysm (sIA) walls
From: Lymphatic vessels are present in human saccular intracranial aneurysms
LEC marker* | sIAs positive for LEC marker | Location in the sIA wall | Cellular /extracellular location | sIAs with lymphatic vessels | Staining pattern within thrombus | Colocalization with LYVE-1 staining in consecutive sections |
|---|---|---|---|---|---|---|
LYVE-1 | 30/36 (83%) | Adventitial; variation in the extent of staining | Cellular and extracellular | 19/36 (52%) | 13/17 (76%) single positive cells; lymphatic vessels in2/17 (12%) | – |
Podoplanin | 32/34 (94%) | Adventitial; large positively stained areas | Cellular and extracellular | 23/34** (68%) | 14/17 (82%) single positive cells and large, diffuse positively stained areas; lymphatic vessels in 3/17 (18%) | Complete in 8/29 (28%) and partial in 18/29 (62%) samples positive for both stainings |
VEGFR-3 | 36/36 (100%) | Scattered positive cells in all samples, wide positively stained adventitial areas in 4/36 (11%) samples | Cellular and extracellular | 11/36 (30%) | 17/17 (100%) large positively stained areas; lymphatic vessels in 0/17 (0%) | Wide positively stained areas in the adventitial side of the wall in 4/36 (11%) samples colocalized completely with the LYVE-1-positive staining |
Prox1 | 26/36 (72%) | Adventitial; small positively stained areas | Cellular and extracellular;positive nuclei in 12/36 samples | 8/36 (22%) | 12/17 (71%) single positive cells and small, diffuse positively stained areas; lymphatic vessels in 0/17 (0%) | Complete in 2/23 (9%) and partial in 13/23 (56%) samples positive for both stainings |