Fig. 4

CD4⁺ T cells control iNPC LGG formation in a Cxcl10-dependent manner a Mouse strains harboring NF1-null iNPC-derived LGGs at 1 mpi. b Schematic detailing the experimental design. c Heat map analysis of RNA sequencing performed on whole brainstem tissues from naïve wild type (WT) and Rag1^−/− mice reveals segregation of transcript expression. d List of > 3-fold differentially expressed transcripts from the brainstems of Rag1^−/− mice relative to WT controls. e Relative expression (R.E.) of Cxcl10 transcripts in WT and immunodeficient (CD8-deficient, Rag1^−/−, NOD/SCID, CD4/8-deficient and CD4-deficient) mice. n = 5; data are represented as means ± SEM; one-way ANOVA with Bonferroni post-test correction. Individual p values are indicated above each bar. ns, not significant. f Relative Cxcl10 expression is reduced in Rag1^−/− astrocytes compared to WT controls. n = 3; data are represented as means ± SEM, two-tailed student’s t-test; p = 0.0089. g Schematic of the experimental design, and histogram demonstrating that Cxcl10 protein levels are increased by 6.5- and 24.4-fold in Rag1^−/− astrocytes treated with CD8⁺ and CD4⁺ T cell conditioned media (TCM), respectively, relative to untreated Rag1^−/− astrocytes. n = 6; one-way ANOVA with Bonferroni post-test correction; individual p values are indicated above each bar