Fig. 1

Rag1^−/− mice develop NF1-null and KIAA1549:BRAF-expressing low-grade gliomas (LGGs) a Injection of NF1-null iNPCs (2041C > T^−/−, 6513T > A^−/−) into the brainstems or KIAA1549:BRAF-expressing iNPCs into the cerebella of Rag1^−/− mice result in the formation of LGGs detectable by MRI (denoted by red dotted lines). b Summary of injected Rag1^−/− mice harboring iNPC LGGs. c LGGs are hypercellular (H&E staining) and immunopositive for Ku80 (human-specific antibody), Ki67, glial (GFAP, OLIG2) and neuronal (synaptophysin) marker expression, by 1 month post-injection (mpi). The tumors had both glial only (top panels) and mixed embryonal/glial (lower panels) histological characteristics. Scale bars: left, Ku80/ H&E panels, 1 mm; other panels, 100 µm. d LGGs contain CD133⁺, ABCG1⁺, PDPN⁺, BLBP⁺ and GFAP⁺ cells (white arrowheads). No Ku80⁺ LGG tumor cells had SOX10 or p16 expression. Scale bar, 100 µm