Fig. 2
SARS-CoV-2 in nasal turbinates, olfactory bulb, cerebral cortex and cerebellum. Syrian golden hamsters were intranasally inoculated with 105TCID50 D614G, 5.0 × 104PFU Delta and Omicron BA.1 variant. Five days after inoculation, hamsters were sacrificed. Infectious virus titers (A) and viral copy RNA (B) in homogenates of nasal turbinates, olfactory bulb, cerebral cortex and cerebellum were quantified using plaque assay or reverse transcriptase quantitate PCR (RT-qPCR) respectively. Statistical significance was calculated with a Two-Way analysis of variance (ANOVA) with a Dunnett’s posthoc test. Averaged values of four individual animals per infection group were compared with all every other averaged value of four infected animals. Asterisks indicate statistical significance*, P < 0.05. LOD, limit of detection .(C) Hematoxylin and eosin (H&E) staining and SARS-CoV-2 nucleoprotein detected using immunohistochemistry (S2 IHC) in the olfactory mucosa of hamsters five days post exposure. D H&E staining SARS-CoV-2 nucleoprotein (S2 IHC) and SARS-CoV-2 viral RNA (S2 in-situ hybridization ISH) in the glomerular layer of the olfactory bulb in D614G inoculated hamsters