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Table 2 Characterization and quantification of p62-immunopositive spinal cord motor neuron (MN) inclusions, as well as diffuse cytoplasmic and nuclear p62 staining, in seven micron paraffin-embedded formalin-fixed tissue sections of the cervical and thoracic spinal cord from all post-mortem tissue cases

From: Co-deposition of SOD1, TDP-43 and p62 proteinopathies in ALS: evidence for multifaceted pathways underlying neurodegeneration

Case #

Diagnostic group

p62 morphology

Quantification (%MNs)

Globular

Skein-like

Punctate

Diffuse

Incl./diff

Nuclear

1

Age-matched control

NA

NA

NA

NA

NA

NA

2

Age-matched control

NA

NA

NA

NA

NA

NA

3

Age-matched control

NA

NA

NA

NA

NA

NA

4

Age-matched control

−

−

−

−

−

+

5

Age-matched control

−

−

−

−

−

+

6

Age-matched control

−

−

−

√

+

+

7

Age-matched control

−

−

−

−

−

+

8

Age-matched control

−

−

−

√

+

+

9

Age-matched control

−

−

−

−

−

+

10

Age-matched control

√

√

−

√

+

+

11

fALS (SOD1, I113T)

√

−

√

√

++++

−

12

fALS (SOD1, I113T)

−

−

√

−

+++

−

13

fALS (SOD1, D101G)

√

−

√

−

++++

−

14

fALS (C9ORF72, 30+positive)

√

√

−

√

++++

−

15

fALS (unknown mutation)

√

√

−

√

++++

−

16

fALS (C9ORF72, 30+positive)

√

√

−

√

++++

+

17

fALS (C9ORF72, 30+positive)

√

√

√

√

+++

−

18

sALS

√

−

√

−

++++

−

19

sALS

√

−

−

−

++++

−

20

sALS

√

√

−

√

++

−

21

sALS

√

√

−

√

+++

−

22

sALS

NA

NA

NA

NA

NA

NA

23

sALS

√

√

−

√

+++

−

24

sALS

√

√

−

√

++++

−

25

sALS

√

√

√

√

+++

−

26

sALS

NA

NA

NA

NA

NA

NA

  1. Fixed tissues were not available (NA) for some cases. The four morphologies of p62 pathology were noted as present (√) or absent (−), and the proportion of motor neurons exhibiting any of the four morphologies quantified. The proportion of motor neurons exhibiting nuclear p62 immunostaining was also quantified. Quantification classifications; − = 0%, + =  > 0–25%, ++26–50%, +++ = 51–75%, ++++ = 76–100%