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Table 2 Characterization and quantification of p62-immunopositive spinal cord motor neuron (MN) inclusions, as well as diffuse cytoplasmic and nuclear p62 staining, in seven micron paraffin-embedded formalin-fixed tissue sections of the cervical and thoracic spinal cord from all post-mortem tissue cases

From: Co-deposition of SOD1, TDP-43 and p62 proteinopathies in ALS: evidence for multifaceted pathways underlying neurodegeneration

Case # Diagnostic group p62 morphology Quantification (%MNs)
Globular Skein-like Punctate Diffuse Incl./diff Nuclear
1 Age-matched control NA NA NA NA NA NA
2 Age-matched control NA NA NA NA NA NA
3 Age-matched control NA NA NA NA NA NA
4 Age-matched control +
5 Age-matched control +
6 Age-matched control + +
7 Age-matched control +
8 Age-matched control + +
9 Age-matched control +
10 Age-matched control + +
11 fALS (SOD1, I113T) ++++
12 fALS (SOD1, I113T) +++
13 fALS (SOD1, D101G) ++++
14 fALS (C9ORF72, 30+positive) ++++
15 fALS (unknown mutation) ++++
16 fALS (C9ORF72, 30+positive) ++++ +
17 fALS (C9ORF72, 30+positive) +++
18 sALS ++++
19 sALS ++++
20 sALS ++
21 sALS +++
22 sALS NA NA NA NA NA NA
23 sALS +++
24 sALS ++++
25 sALS +++
26 sALS NA NA NA NA NA NA
  1. Fixed tissues were not available (NA) for some cases. The four morphologies of p62 pathology were noted as present (√) or absent (−), and the proportion of motor neurons exhibiting any of the four morphologies quantified. The proportion of motor neurons exhibiting nuclear p62 immunostaining was also quantified. Quantification classifications; − = 0%, + =  > 0–25%, ++26–50%, +++ = 51–75%, ++++ = 76–100%