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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Co-deposition of SOD1, TDP-43 and p62 proteinopathies in ALS: evidence for multifaceted pathways underlying neurodegeneration

Fig. 5

Coincidence and colocalization of disSOD1, pTDP-43 and p62 pathologies in spinal cord motor neurons of non-SOD1-fALS and sALS cases. a Immunostaining for disordered SOD1 (disSOD1), phosphorylated TDP-43 (pTDP-43) and p62 proteins using fluorescence microscopy in ventral spinal cord motor neurons of non-SOD1-fALS and sALS cases. We identified diffuse cytoplasmic staining (arrows), as well as punctate (dotted arrowheads), globular (single downwards arrowheads) and fibrillar skein-like (double downwards arrowheads) inclusions, comprised of one or more proteins of interest in these neurons. Rows represent each of the different combinations of pathologies observed in motor neurons of that subgroup; disSOD1 (magenta), pTDP-43 (blue) or p62 (cyan) deposition alone, disSOD1 and pTDP-43 (light pink), disSOD1 and p62 (orange), or pTDP-43 and p62 (light blue) co-deposition, or the co-deposition of all three pathologies (white). Scale bars in represent 25 µm. Antibody details are listed in Additional file 1: Table S3. No immunostaining was observed in spinal cord tissue sections processed as above in the absence of primary antibodies (Additional file 1: Figure S1). b A venn diagram illustrating the proportion of the total motor neurons examined (non-SOD1-fALS, n = 104; sALS, n = 123) which exhibit each of the different individual or combined pathologies. Each segment corresponds to a row in panel a, and are pictured next to their corresponding row. Where co-deposition of two pathologies was observed within a motor neuron, we noted the presence (colocalized, grey) or absence (coincidental, black) of spatial overlap

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