Fig. 6

Molecular alterations in the rat brain following the AβO infusion. A Quantification of the levels of amyloid peptides (left panel) and pTau (right panel) in rat hippocampal tissues from AD and control animals (n = 6 per group) using ELISAs. B IHC with the 6E10 antibody showed no plaques in the brains of AD rats. The same antibody strongly stained Aβ plaques in the brains of 28-month-old TgF344-AD rat, which were used as a positive control. Representative images of CA1 and DG regions of WT rats injected with AD human brain tissue extract (top panels) and Tg rats (bottom panels) are shown. Scale bar, 300 µm. C The relative mRNA expression of early AD markers in AD and control animals (n = 6 per group) measured using qPCR. Data were normalized to two reference genes: ACTB and RPL13A. Data are presented as the means ± SEM. *p < 0.05, **p < 0.01