Fig. 3

Treatment with PMX205 showed a beneficial effect in reducing the dystrophic neurites pathology on the Tg2576 Alzheimer’s mice. A Representative images of amyloid plaques (ThioS: green), dystrophic neurites (Lamp1: red) and protofibrillar amyloid species (OC: blue) in the hippocampus of Tg2576 mice treated with vehicle (A1) or PMX205 (A2). Quantitative analysis of Lamp1 showed a significant reduction in the appearance of dystrophic neurites in mice treated with PMX205 in both hippocampus and cortex (A3 and A6). Analysis of the ratio Lamp1/ThioS showed no difference between vehicle and PMX205 treated mice (A4 and A7). Pearson’s correlation test showed a positive correlation of Lamp1 with ThioS + plaques in the hippocampus (R2 = 0.87; A5) and cortex (R2 = 0.71; A8). Data are shown as Mean ± SEM and normalized to control group (Tg2576-vehicle). Statistical analysis used a two-tailed t-test and Pearson’s correlation test. Significance is indicated as *p < 0.05; **p < 0.01; and ***p < 0.001. n = 4–5 per group. Scale bar: A1–A2: 100 µm