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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Unraveling axonal mechanisms of traumatic brain injury

Fig. 7

Adaptation of the axonal transport in response to the injury. A and B Tracking of APP-GFP particles in pre, during and post-injury stages (A, scale bar: 10 µm). Proportion of moving and stationary particles in the full tracking time (pre, injury and post injury stages) (B). Anterograde, retrograde and stationary movement particle proportions for each stage (n = 4). Anterograde and retrograde median velocities in the three different stages (n = 33–56). C Sequence of an APP-GFP particle moving anterogradely through an axonal swelling (scale bar: 5 µm). D ELISA Aβ40 and Aβ42 quantification in the axonal compartment comparing control and injured axons (n = 3). E and F Tracking of Synaptophysin-GFP particles in pre, during and post-injury stages (E, scale bar: 10 µm). Proportion of moving and stationary particles in the full tracking time (F). Anterograde, retrograde and stationary movement particle proportions for each stage (n = 4). Anterograde and retrograde median velocities in the three different stages (n = 13–40). G and H Tracking of Mito-GFP labeled mitochondria in pre, during and post-injury stages (G, scale bar: 10 µm). Proportion of moving and stationary particles in the full tracking time (H). Anterograde, retrograde and stationary movement particle proportions for each stage (n = 5). Anterograde and retrograde median velocities in the three different stages (n = 21–40). I Mitochondrial membrane potential levels during injury measured with TMRE probe (n = 7). Data are mean ± SEM (*p < 0.05, **p < 0.01). Statistical comparisons were performed using student t-test (D), two-way ANOVA followed by Dunnett’s multiple comparison test against stationary group (proportions: B, F and H) or Kruskal–Wallis test followed by Dunn’s multiple comparisons test against pre-injury (speed: B, F and H)

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