Fig. 5

Phosphorylation changes of the axonal proteins following injury. A Axonal and neuronal compartment fractions (A/N) in both control and injury treatments (C/I) were enriched for phosphorylation and processed for quantitative mass spectrometry. Heatmaps of phospho-peptides levels comparing injury versus control treatment for both axonal and neuronal fractions (n = 4). B Volcano plots of phosphopeptides levels comparing injury versus control treatment for both the axonal and neuronal fractions. C Pie charts depict the number of identified proteins that significantly increase or decrease their phosphorylation and the number of proteins that are not modified after injury. D Enrichment analysis (GO Biological Processes) of the proteins that are significantly regulated by phosphorylation after injury, using as background all the detected phosphoproteins. Comparison of significant terms between the axonal and neuronal fractions. E Stringplot showing all the phosphoproteins in the axonal fraction significantly changed after injury. F Overrepresentation analysis of the proteins in (E) showing top significant terms for GO molecular functions. G Kinome tree highlighting predicted kinases in both fractions, based on the significantly changed phosphopeptides as substrates (left). List of kinases and their families with the predicted activity based on their substrate’s fold change. H Stringplots of phosphoproteins present in specific cytoskeletal terms found in (F) and their kinases predicted activity