Fig. 6
From: Pathological changes induced by Alzheimer’s brain inoculation in amyloid-beta plaque-bearing mice
Synaptic density is decreased in ADbe-inoculated APPswe/PS1dE9 mice and correlates with tau pathology in neuritic plaques (8 mpi). Representative image of Bassoon (red) and Homer1 (green) immunolabelling in Ctrlbe- (a, b) and ADbe-inoculated (c, d) animals. (non-segmented confocal optical microscopic images (a, c); segmented images of insets (b, d). Bassoon/Homer-positive puncta (yellow in a–d) were quantified as an index of synaptic density. The synaptic density decreased in perirhinal/entorhinal cortex of ADbe mice compared to Ctrlbe-inoculated mice at 8 mpi (e, p = 0.002). Synaptic density was inversely correlated with tau pathology in neuritic plaques in the perirhinal/entorhinal cortex (f: neuritic plaque count; g: AT8-positive area within neuritic plaques), but not with neuropil threads (h), neurofibrillary tangles (i), or Aβ pathology (j). Spearman’s correlations with significance level at p < 0.05. nCtrl = 15, nAD1 = 15 (light pink), nAD2 = 20 mice (dark pink). **p < 0.01; Data are shown as mean ± s.e.m