Fig. 2

Quantification of nuclear phospho-S129 aSyn in temporal cortex of DLB and Control cases. Example of 60 × objective lens confocal images of lateral temporal cortex stained with phospho-serine 129 aSyn (pS129; EP1536Y) and NeuN antibodies with a DAPI nuclear co-stain (a). Images from a control (Con) and dementia with Lewy body (DLB) case are shown. Note prominent pS129 nuclear stain in both cases, with additional cytoplasmic pathology in the form of Lewy neurites in the DLB case. Quantification of nuclear pS129 immunoreactivity in control and DLB cases as segregated according to NeuN reactivity is shown (b) alongside correlative analysis of nuclear pS129 levels between NeuN positive (NeuN +ve) and negative (NeuN −ve) nuclei (c), with spearman’s correlation (r), given when analysed as a combined total single cohort or when divided into Con and DLB. Frequency plots of individual NeuN +ve (i) and NeuN −ve (ii) nuclei are additionally shown (d), reporting the % nuclei of con and DLB cases within a of 20 arbitrary units. Data in c) reported as scatter plots of mean fluorescence arbitrary units per case with group mean ± SEM indicated and in d) also as mean fluorescence units, dotted line demonstrates best-fit line when all cases (con + DLB) are considered combined. ** = p < 0.01. Scale bar in a = 50 µm