Fig. 7

Soleus NMJs show significant decrease in localization of NFH to the axon terminal. A, C, E, G To evaluate localization of cytoskeletal structural element NFH to the axon terminal, NMJs from tibialis anterior were stained with α-BTX, synaptophysin, and SMI31 (phospho-NFH) or α-BTX, TUJ1 (BIII-tubulin, and SMI32 (unphospho-NFH) and evaluated for colocalization with the synaptophysin or α-BTX, respectively. B Transgenic mice showed no changes in uNFH localization to the terminal; however, knock-in mice showed a significant decrease in uNFH localization with the terminal (D; p < 0.05). D In contrast, pNFH colocalization with the terminal was significantly decreased in transgenic mice (E; p < 0.01) but not in knock-in mice (F). Mann–Whitney Test was used to evaluate statistical significance. uNFH Unphosphorylated neurofilament heavy chain; pNFH Phosphorylated neurofilament heavy chain; NTg Non-transgenic; Tg Transgenic; WT Wild-type; KI Knock-in