Fig. 3
GR phosphorylation influences dendritic spines plasticity as a function of amyloidogenesis. a Field of view imaged 3 times in triple transgenic mice. b Remodeling of dendritic spines between time points as a function of distance to the nearest amyloid plaque centered in the dendrospinogram. Yellow circle shows the zone at 25-to-60 μm from the amyloid plaque. c Quantitative dynamics proximal and distal of amyloid plaques. Means ± SEM of N(3,6,9 months) = 9,9,7 Nr3c1+/+-APP/PS1 and 10,10,5 Nr3c1ki/ki-APP/PS1 mice. Three-way ANOVA: effect of genotype on formation F1,34 = 3.95, P = 0.05 and elimination F1,17 = 6.67, P = 0.08; effect of distance on formation F1,34 = 13.2, P = 0.0009 and elimination F1,17 = 13.4, P = 0.002; effect of aging on formation F1,21 = 0.12, P = 0.7 and elimination F1,17 = 2.9, P = 0.1 post-hoc Tukey test comparing distances #P < 0.05. d Survival of dendritic spines proximal and distal of amyloid plaques. Old spines (means ± SEM) of N(3,6,9 months) = 10,10,8 Nr3c1+/+-APP/PS1; 10,10,5 Nr3c1ki/ki-APP/PS1 mice. Three-way ANOVA: effect of APP/PS1 F1,3 = 5.1, P = 0.1; effect of distance F1,18 = 7.7, P = 0.01 post-hoc Tukey test comparing distances #P < 0.05 and genotypes *P < 0.05. New spines (Quartiles and median of dataset from Min-to-Max) of N(<25,>25μm) = 4,7 Nr3c1+/+-APP/PS1 and 5,5 Nr3c1ki/ki-APP/PS1 mice. Two-way ANOVA: effect of genotype F1,17 = 0.001; effect of distance F1,17 = 21.3, P = 0.0002 post-hoc Tukey test comparing distances #P = 0.02, ##P = 0.003. e Spine clustering is greater than chance level distal of amyloid plaques. Mean ± SEM of N(+/+; APP/PS1, ki/ki;APP/PS1) = 6, 8, 8 mice. Two-tailed unpaired t-test comparing observed and simulated *P < 0.05, distal and proximal #P < 0.001 or genotypes.¶P = 0.04