Fig. 4
From: Reassessment of Neuronal Tau Distribution in Adult Human Brain and Implications for Tau Pathobiology
Comparative biochemical fraction analyses of gray and white matter from the temporal cortex of AD and control cases with anti-tau antibody 1B1. Biochemical fractionation of AD and CTL cerebral temporal cortex was performed as described in Materials and Methods. Equal amount of protein (5Â \(\upmu\)g) from the high salt (HS) soluble, Triton/HS soluble, Sarkosyl/HS soluble, and Sarkosyl-insoluble SDS/urea soluble fractions were separated by SDS-PAGE and analyzed by immunoblotting with tau antibody, 1B1. The HS fractions were also probed for GAPDH and the SDS/urea fractions were probed for NFL. The mobility of protein markers with their molecular masses are indicated on the left side of each immunoblot