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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Reassessment of Neuronal Tau Distribution in Adult Human Brain and Implications for Tau Pathobiology

Fig. 1

Characterization of the specificity of the new tau antibodies 1B1 and 1H11. a Schematic of full-length human tau (2N4R) with the location of the antibody epitopes used in the study. Created with BioRender.com. b Amino acid sequence of human and mouse tau corresponding to residues 19 to 38 in human tau. Non-homologous amino acids between species are indicated in black lettering. Dashes indicate residues that are not present in mouse tau. c 10 µg of total brain lysate from tau KO, nTg, and PS19 mice were resolved by SDS-PAGE as indicated above each lane and used for immunoblotting as described in Materials and Methods. Blots were probed with antibodies 3026 (mouse and human tau), 1B1 (human tau), 1H11 (human tau), CP27 (human tau) and anti-GAPDH as a loading control. The mobility of protein markers with their molecular masses are indicated on the left side of each immunoblot. d IHC was performed as described in Materials and Methods. Brains of tau KO, nTg, and PS19 mice were stained with the total tau antibody Tau-5, and the two novel monoclonal total human tau antibodies, 1B1 and 1H11. Sections were counterstained with hematoxylin. Scale bar indicates 300 \(\mu \mathrm{m}\)

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