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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Visual imaging as a predictor of neurodegeneration in experimental autoimmune demyelination and multiple sclerosis

Fig. 4

Retinal nerve fiber and ganglion cell/inner plexiform layer thicknesses are decreased during chronic EAE, while VEPs are delayed during acute and chronic EAE. a Representative SLO images. Scale bars = 200 µm. P values listed consecutively are baseline vs 15, 35, or 60 dpi, respectively b Quantification of RNFL area (P = 0.1020, **P = 0.0012, ****P < 0.0001). c Representative OCT b scans. d–h Quantification of the thicknesses of the RNFL (P = 0.7494, ****P < 0.0001, ****P < 0.0001), GC/IPL (P = 0.1083, **P = 0.0017, **P = 0.0014), INL (P = 0.1795, P = 0.8098, P = 0.8662), OPL (P = 0.8955, P = 0.5683, P = 0.9095), and ONL (P = 0.7659, P = 0.7080, P = 0.8720). i–k Correlation analyses between EAE clinical score (area under curve for each group) and RNFL thickness (i, Spearman r = − 0.7922, ****P < 0.0001), EAE clinical score versus GC/IPL thickness (j, Spearman r = − 0.6676, ****P < 0.0001), and EAE clinical score versus Brn3a+ cells in the retina (k, Spearman r = − 0.6070, **P = 0.0013) across all time points. l–m VEP latency (implicit time) and amplitude. P values for VEP data are listed in Table S1. Statistical differences were determined using a one-way ANOVA with Holm-Šidák post-hoc test (at each flash luminance for VEP) or Spearman r test for correlation analyses. All data are expressed as means ± SEM including n = 6–10 mice each for baseline and 15, 35, and 60 dpi

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