Fig. 5

Comparison of profiles between FTD-MAPT and AD demonstrates presence of FTD subtype-specific and general neurodegenerative protein signatures. A EWCE analysis of lower (n = 406) and higher (n = 556) expressed proteins in temporal cortical AD vs NDC shows astrocyte, endothelial cell, and microglial cell enrichment (all p = 0) for higher expressed proteins, and excitatory and inhibitory neuron enrichment (both p = 0) for lower expressed proteins. Comparison with EWCE results for FTD-MAPT confirms distinct involvement of oligodendrocytes in FTD-MAPT. B Overlay of differentially expressed proteins shared between FTD-MAPT and AD (n = 195) on the protein profile for FTD-MAPT shows that the majority of shared proteins have the same direction of differential expression. C GO analysis on shared proteins. D EWCE analysis on shared proteins. Significant enrichment is seen for astrocytes (p = 0) for higher expressed proteins, and for excitatory (p = 0) and inhibitory (p = 0.002) neurons for lower expressed proteins. E GO analysis on proteins that are only differentially expressed in FTD-MAPT (n = 259). ‘RNA processing’, ‘Ion transport’, ‘Axon’ and ‘Neuron’ GO groups are confirmed to be distinct for the FTD-MAPT subtype when compared with AD. F EWCE analysis on proteins only differentially expressed in FTD-MAPT. Significant enrichment is seen for astrocytes (p = 0) and OPCs (p = 0.0074) for higher expressed proteins, and for excitatory neurons and oligodendrocytes (both p = 0) for lower expressed proteins. EWCE analysis is performed using bootstrapped t-test statistics with multiple testing correction by the Benjamini–Hochberg method. For GO analysis, only classical GO terms (BP/CC/MF) are taken into account and only ‘Best Per Parent’ GO terms are shown. The number of proteins in each GO term is listed. GO terms are further categorized into GO groups. Biosynth.; biosynthesis, Exc.; excitatory, Develop.; development, Inh.; Inhibitory, Ion tr; ion transport, Neg. reg.; negative regulation, Polym.; polymerase, s.d.; standard deviation, Transmiss.; transmission