Fig. 1

RiMOD-FTD study workflow. After tissue preparation of all 66 brain cortical tissue samples, we performed DIA mass spectrometry (SWATH), followed by differential protein expression analysis. The identified proteins with differential expression in the FTD subtypes were further subjected to downstream bioinformatics analyses. These included (1) expression-weighted cell type enrichment based on existing single cell RNAseq (scRNAseq) data resources, (2) several gene ontology analyses, and (3) an extensive comparison with an in-house sporadic AD proteomic data set. The results of these analyses were integrated and led to the identification of distinct cell type-specific protein signatures in GRN and MAPT genetic FTD subtypes