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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Modulation of synaptic plasticity, motor unit physiology, and TDP-43 pathology by CHCHD10

Fig. 3

Long-term synaptic plasticity deficits in CHCHD10R15L and CHCHD10S59L transgenic mice. a Equal protein amounts subjected to biochemical fractionation for synaptosomes and cytosol from 10-month-old WT, CHCHD10WT, and CHCHD10R15L mice and immunoblotted for the indicated proteins. b Representative images of brain sections from 10-month-old CHCHD10WT, CHCHD10R15L, and CHCHD10S59L mice immunostained for synaptophysin (red). Small white boxes magnified in upper white boxed insets. c Quantification of synaptophysin intensity in hippocampus CA3 region from Fig. (3c) (1-way ANOVA – synaptophysin: F(3, 94) = 54.39, P < 0.0001: posthoc Tukey, *p < 0.05, #p < 0.001, ##p < 0.0001, n = 21–27 sections/genotype from 4 mice/genotype). d Schematic of short-term and long-term synaptic plasticity recordings from brain slices. e-g Brain slices from 10-month-old CHCHD10WT, CHCHD10R15L, and CHCHD10S59L mice subjected to measurement of (e) input–output curve by stepping up stimulation amplitude from 0.5 to 18 mV (2-way ANOVA, genotype F(3, 4025) = 18.9, P < 0.0001: posthoc Tukey, not significant n = 25–32 slices/genotype from 4 mice/genotype), (f) PPF as a function of short-term plasticity from 20 to 300 ms interstimulus intervals (2-way ANOVA, genotype F(3, 1770) = 8.878, P < 0.0001: posthoc Tukey, *p < 0.05, ##p < 0.0001, n = 26–38 slices/genotype from 4 mice/genotype), and (g) LTP induced by theta burst stimulation (2-way ANOVA, genotype F(3, 9600) = 935.9, P < 0.0001: posthoc Tukey, ##p < 0.0001, n = 28 to 38 slices/genotype from 4 mice/genotype)

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