Table 2 Antibody characteristics
From: Neurogenesis and neuronal differentiation in the postnatal frontal cortex in Down syndrome
| Antigen | Primary antibody | Dilution | Company Cat. # | Secondary Antibody (Company) |
|---|---|---|---|---|
| SMI-32* | Mouse monoclonal to anti-Neurofilament H, non-phosphorylated |
1:500 1:50* |
Biolegend 801701 |
Biotinylated goat anti-mouse IgG (Vector Laboratories) Cy3 donkey anti-mouse IgG (Jackson Immunoresearch Laboratories)* |
| APP/Aβ | Mouse monoclonal to residues 1–16 of Aβ (6E10) | 1:300 |
Biolegend 803002 | |
| Parvalbumin | Mouse monoclonal anti-parvalbumin | 1:500 |
Millipore MAB1572 | |
| Doublecortin | Mouse monoclonal neuronal migration protein DCX E-6 | 1:250 |
Santa Cruz Biotechnology sc-271390 | |
| CP13 | Mouse monoclonal phospho-tau (Ser202) | 1:100 | Gift from Peter Davies | |
| PHF-1 | Mouse monoclonal phospho-tau (Ser396/Ser404) | 1:100 | Gift from Peter Davies | |
| Ki-67 | Mouse monoclonal to human MIB-1 | 1:500 |
Dako M7240 | |
| Calbindin D28-K* | Rabbit polyclonal to 28 kD calcium-binding protein |
1:1000 1:75* |
Swant CB38 |
Biotinylated goat anti-rabbit IgG (Vector Laboratories) Cy5 donkey anti-rabbit (Jackson Immunoresearch Laboratories)* |
| Calretinin | Rabbit polyclonal to 99 aa epitope from the internal region of rat calretinin | 1:500 |
Millipore AB5054 | |
| Aβ1–42 | Rabbit polyclonal to 6 aa peptide sequence from C-terminus of human Aβ1–42 | 1:100 |
Millipore AB5078P |