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Table 2 Antibody characteristics

From: Neurogenesis and neuronal differentiation in the postnatal frontal cortex in Down syndrome

Antigen Primary antibody Dilution Company Cat. # Secondary Antibody (Company)
SMI-32* Mouse monoclonal to anti-Neurofilament H, non-phosphorylated 1:500
1:50*
Biolegend
801701
Biotinylated goat anti-mouse IgG (Vector Laboratories)
Cy3 donkey anti-mouse IgG (Jackson Immunoresearch Laboratories)*
APP/Aβ Mouse monoclonal to residues 1–16 of Aβ (6E10) 1:300 Biolegend
803002
 
Parvalbumin Mouse monoclonal anti-parvalbumin 1:500 Millipore
MAB1572
 
Doublecortin Mouse monoclonal neuronal migration protein DCX E-6 1:250 Santa Cruz
Biotechnology
sc-271390
 
CP13 Mouse monoclonal phospho-tau (Ser202) 1:100 Gift from Peter Davies  
PHF-1 Mouse monoclonal phospho-tau (Ser396/Ser404) 1:100 Gift from Peter Davies  
Ki-67 Mouse monoclonal to human MIB-1 1:500 Dako
M7240
 
Calbindin D28-K* Rabbit polyclonal to 28 kD calcium-binding protein 1:1000
1:75*
Swant
CB38
Biotinylated goat anti-rabbit IgG (Vector Laboratories)
Cy5 donkey anti-rabbit (Jackson Immunoresearch Laboratories)*
Calretinin Rabbit polyclonal to 99 aa epitope from the internal region of rat calretinin 1:500 Millipore
AB5054
 
1–42 Rabbit polyclonal to 6 aa peptide sequence from C-terminus of human Aβ1–42 1:100 Millipore
AB5078P
 
  1. *Immunofluorescence staining