Fig. 9
From: Neurogenesis and neuronal differentiation in the postnatal frontal cortex in Down syndrome
Immunofluorescent images of FC SG and IG layers showing single Calb labeled cells (green) and SMI-32 (red) and dual Calb/SMI32 positive cells (reddish-orange) in 48 (A, B) and 174 (C, D) wk NTD and in 45 (E, F) and 196 (G, H) postnatal wk-old DS cases. Note that double labeled pyramidal Calb/SMI-32 positive cells (white arrows) were mainly observed in the SG layers in the oldest 174 wk NTD (C) and 196 wk DS (G) cases, but not in the youngest cases. Small single Calb-ir positive cells (yellow arrows) were seen in SG and IG layers in 174 wk NTD (C) and 196 wk DS (H), respectively. Blue nuclei were stained with DAPI. Scale bar = 50 µm